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The extract was rotary evaporated at 30 C to a volume of 2 ml, acidified to pH 4 with formic acid, and 200 l aliquots were purified using preparative HPLC (Agilent Technologies, 1260 Infinity) over a Luna 5 m phenyl-hexyl 100 (250 10 mm) column and a 15 min linear gradient from 0% acetonitrile in H 2 O to 16% acetonitrile in H 2 O at 4 ml min 1 flow

The MMS increased after the intervention in 93% in the Relax and in 91% in the Phys group (p = 0.770)

d) sequential coupling of Fmoc-Arg(Pbf)-OF[, Fmoc-Val-OFl, Fmoc-Leu-OFl, Fmoc- Trp(Boc)-OFl, Fmoc-Ala-OFl, Fmoc-Ile-OFl, Fmoc-Phe-OFl, Fmoc-Glu(OtBu)-OFl, Fmoc-Lys(C18diacidmono-t-butyl-Y-Glu(AEEA-AEEA)-OtBu)-OFl, Fmoc-Ala-OFl, Fmoc-Ala-OFl, Fmoc-Gln(Trt)-OFl to the obtained resin in step-c) in presence of a coupling agent

Frequently Asked Questions About DME Treatment Authorization Request Form DME Treatment Authorization Request Form has a Form Complexity Index of 54 out of 100 , placing it in the moderate complexity tier